How to choose the right purity for custom peptides
In research and development using peptides, how much purity should be selected for custom peptide synthesis, and is the purity of the selected peptide the higher the better?
In general, the same sequence, the same number of peptides will vary in price because of its purity, the higher the purity, the higher the price of the peptide per unit quality.
Our company can provide different purity levels for customers to choose from, from crude to >98% purity. According to customer needs, we can provide ultra-pure peptides with purity >98%.
Crude peptides are not recommended for use in biological experiments. Crude peptides may contain large amounts of non-peptide impurities, such as residual organic solvents, scavengers, TFA, and other incomplete peptides. TFA cannot be completely eliminated, and peptides are usually delivered in the form of TFA salts.If residual TFA affects your experiment, we recommend other salt forms such as acetate and hydrochloride. These salts are typically more than 20-30% more expensive than regular TFA salts. This is due to more peptide loss and the need for more raw materials during the conversion process.
We recommend the following levels of peptide purity for various items:
purity | purpose |
70%~79% | Peptide microarray |
As an antigen for the preparation of antibodies | |
Chromatography | |
Antiserum titers were detected by enzyme-linked immunosorbent assay | |
ELISA test | |
Preparation of antigens and purification of polyclonal antibodies | |
Localization of antigenic determinant | |
80%~94% | Enzyme substrate peptide (non-quantitative) |
Western Blotting (non-quantitative) | |
Affinity purification | |
Blocking peptide (non-quantitative) | |
Phosphorylation detection | |
Application of protein electrophoresis and immunocytochemistry | |
In vitro identification | |
Cell attachment study | |
Qualitative enzyme substrate study | |
Phosphorylation study | |
Western blotting method was used to carry out the experiment of protease antagonism | |
95%~97% | Receptor-ligand Interaction (Quantitative) |
Standard ELISA and RIA (Quantitative) | |
Enzyme Studies and Blocking Experiments (Quantitative) | |
Biological assay in vitro and in vivo | |
mass spectrometry (ms) | |
In vivo study | |
In vitro identification | |
Nuclear magnetic resonance study | |
Generating standard curve | |
Antigenic determinant localization | |
Quantitative proteolytic studies | |
Quantitative phosphorylation study | |
Antagonistic and competitive experiments of proteases | |
Quantitative study of receptor and ligand interactions | |
Other quantitative tests | |
≥98% | APIs(Pharmaceutical Active Ingredients) |
SAR research | |
APIs(Pharmaceutical Active Ingredients) | |
clinical test | |
Standard products and standard curve production | |
X-ray crystal study | |
Other sensitive experiments: enzyme-substrate, receptor-ligand interaction, blocking and competition experiments | |
Pharmaceutical peptide | |
Peptides for cosmetics | |
study on structure-activity relationship |
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