Peptide Knowledge Center

Excitation and emission wavelengths of common fluorochrome

Fluorochrome

Excitation(nm)

Emission(nm)

Cy2 TM

489

506

GFP(Red Shifted)

488

507

YO-PRO TM -1

491

509

YOYO TM -1

491

509

FITC

494

518

FluorX TM

494

519

Alexa TM 488

490

520

Rhodamine 110496520
ABI,5-FAM494522

Oregon Green TM 500

503

522

Oregon Green TM 488

496

524

RlboGreen TM

500

525

Rhodamine Green TM

502

527

Rhodamine123

507

529

Magnesium Green TM

506

531

Calcium Green TM

506

533

TO-PRO TM -1

514

533

TOTO?-1

514

533

ABI,JOE

520

548

BODIPY?530/550

530

550

Dil

549

565

BODIPY?TMR

542

568

BODIPY?558/568

558

568

BODIPY?564/570

564

570

Cy3 TM

550

570

Alexa TM 546

555

570

TRITC

547

572

Magnesium Orange TM

550

575

Phycoerythrin,R & B

565

575

Rhodamine Phalloidin

550

575

Calcium Orange TM

549

576

Pyronin Y

555

580

Rhodamine B

555

580

ABI,TAMRA

560

582

Rhodamine Red TM

570

590

Cy3.5 TM

581

596

ABI,ROX

588

608

Calcium Crimson TM

590

615

Alexa TM 594

590

615

Texas Red?

595

615

Nile Red

549

628

YO-PRO TM -3

612

631

YOYO TM -3

612

631

R-Phycocyanin

618

642

C-Phycocyanin

620

648

TO-PRO TM -3

642

660

TOTO?-3

642

660

DiD DilC’(5)

644

665

Cy5 TM

649

670

Thiadicarbocyanine

651

671

Cy5.5

675

69

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