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EDC.HCl | EDAC is an efficient peptide condensing agent and crosslinking agent for peptide synthesis, EDC.HCl It is a kind of water-soluble carbodiimide, which is mainly used as dehydrating agent in the synthesis of peptide, protein and nucleotide, and can realize fast peptide condensation reaction. EDC.HCl It is a water-soluble carbodiimide with linear structure. It is used in the condensation reaction of carboxyl group and primary amine, and has been widely used. For example, amide bond is formed in peptide synthesis, hapten is bound to carrier protein to form immune antigen, and NHS ester with amino reaction activity is formed by reaction with phosphate group at 5 'position of nucleic acid. EDC reacts with amino group to form o-acylurea intermediate which can react with amino group. If the intermediate does not react with amino group, it will hydrolyze quickly and release carboxyl group again. In the presence of n-hydroxythiosuccinimide (sulfo NHS), EDC.HCl The carboxyl group can be converted into sulfo NHS ester with amino reaction activity. In this step, the reaction is carried out by EDC.HCl It can be achieved by mixing the molecules containing carboxyl group and sulfo NHS.
Application: water soluble concentration reagent; general carbonyl activation reagent, used in the process of amide and secondary amine bonding; can react with phosphate group; used in peptide synthesis; protein and nucleic acid cross-linking; preparation, such as immune cross-linking, under typical conditions, pH Under special conditions, we must avoid using amine and carboxylate buffer, water-soluble peptide coupling agent, carbonyl modification in protein and ester synthesis.It is mainly used as Dehydrator in the synthesis of peptide, protein and nucleotide.
Product Data Sheet
Product Name: EDC·HCl / EDAC.HCL
Other name :
Molecular Formula： C8H17N3•HCL
Molecular Weight： 191.7
Character: white crystal
Melting point: 104 ~ 114 ℃ (Dec.)
Density : 0.877 g / ml at 20 ° C
Refractive index : 1.461
Shipping & Storage
5 ℃, pay attention to drying.
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