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Biological effects of angiotensin (1-7) (1)

In recent years, due to the rapid development of modern scientific research methods, molecular biology, genomics, biochemistry and other basic disciplines, people have a more comprehensive understanding of the gene structure, protein level, biological function and regulatory mechanism of the renin-angiotensin system (RAS). Since it was discovered in 1988 that angiotensin (Ang) (1-7) can stimulate the secretion of antidiuretic hormone, with the deepening of research, it has been confirmed that as an endogenous antagonist of Ang11 (fmoc protected amino acids supply), it can play a variety of biological effects that are diametrically opposite to Ang11 by binding with Mas receptor, and can antagonize the activity of Ang Ⅱ in vivo and in vitro.

Ang - (1-7) is a heptapeptide (Asp Arg Val Tyr lle Hi Pro) composed of seven amino acid residues (histidine, tyrosine, aspartic acid, valine, proline, arginine, isoleucine, etc.) with a molecular formula of c H N O and a relative molecular weight of 899.0. At present, Ang - (1-7) has the following three pathways in vivo.

 1. AnglI transformation and generation pathway: This is the main generation pathway of Ang - (1-7). Prolyl endopeptidase or neutral endopeptidase acts on AngI of 10 peptides to remove 3 amino acid residues on the peptide chain of proline at position 7 and phenylalanine at position 8, thus generating Ang - (1-7) of heptapeptide.

 2.Angl transformation pathway: under the direct action of prolyl carboxypeptidase, prolyl endopeptidase or angiotensin converting enzyme 2 (ACE2), AnglI of 8 peptides removes an amino acid residue to generate Ang - (1-7)

Ang (1-7) is mainly distributed in the blood, heart, kidney, blood vessels, etc. Its half-life in plasma is short, only 9~10 seconds. Its degradation is mainly carried out in the lung basement membrane and renal cortex, and can be split into non bioactive fragments by aminopeptidase or ACE. ACE pathway in vivo is the main inactivation mode of Ang - (1-7) in circulation and tissues.

Influence of Ang - (1-7) on blood vessels-custom peptide synthesis price list

Anti vascular smooth muscle cell proliferation Ang - (1-7) combines with the receptor under the stimulation of related factors to promote the release of prostaglandins (PGE, PGE, PGI) and prostacyclin, thus starting its anti cell proliferation effect. Ang - (1-7) can play the role of anti cell proliferation, which is closely related to the following factors: ① promoting the release of nitric oxide (NO) and prostaglandins from the body; ② Synergically strengthen various functions of bradykinin (BK). Ang - (1-7) has the effect of anti proliferation of human lung squamous cell carcinoma cells, but does not affect the activity of cyclooxygenase l. Its possible mechanism is to down regulate the expression of cyclooxygenase 2 mRNA and its protein.